SIRT3 Stable Expression Cell Line (NCI-H716)

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  • 细胞图0 SIRT3 Stable Expression Cell Line (NCI-H716)-细胞图0
  • 细胞图1 SIRT3 Stable Expression Cell Line (NCI-H716)-细胞图1
  • 质粒图0 SIRT3 Stable Expression Cell Line (NCI-H716)-质粒图0
  • SIRT3 Stable Expression Cell Line (NCI-H716) SIRT3 Stable Expression Cell Line (NCI-H716)-产品图
细胞图0 SIRT3 Stable Expression Cell Line (NCI-H716)-细胞图0
细胞图1 SIRT3 Stable Expression Cell Line (NCI-H716)-细胞图1
质粒图0 SIRT3 Stable Expression Cell Line (NCI-H716)-质粒图0
SIRT3 Stable Expression Cell Line (NCI-H716) SIRT3 Stable Expression Cell Line (NCI-H716)-产品图
SIRT3 Stable Expression Cell Line (NCI-H716) Copy Product Info
Catalog#： YOE-L012
Size： 1*10^6
Culture method： 90%RPMI-1640+10%FBS
STR Report
Datasheet (Use Instruction)
  • 细胞图0 SIRT3 Stable Expression Cell Line (NCI-H716)-细胞图0
  • 细胞图1 SIRT3 Stable Expression Cell Line (NCI-H716)-细胞图1
  • 质粒图0 SIRT3 Stable Expression Cell Line (NCI-H716)-质粒图0
  • SIRT3 Stable Expression Cell Line (NCI-H716) SIRT3 Stable Expression Cell Line (NCI-H716)-产品图
SIRT3 Stable Expression Cell Line (NCI-H716) Copy Product Info
Catalog#： YOE-L012
Size： 1*10^6
Culture method： 90%RPMI-1640+10%FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog YOE-L012 Transcript NM_001370310.1
Cell name SIRT3 Stable Expression Cell Line (NCI-H716) Species Human
Gene Name SIRT3 Passage ratio 1：2-1：3
Cell Morphology grow in cluster form, suspension cell Antibiotic Concentration Puro=1.5μg/ml
Culture method 90%RPMI-1640+10%FBS Mycoplasma test negative
Catalog
YOE-L012
Cell name
SIRT3 Stable Expression Cell Line (NCI-H716)
Gene Name
SIRT3
Cell Morphology
grow in cluster form, suspension cell
Passage ratio
1：2-1：3
Transcript
NM_001370310.1
Species
Human
Antibiotic Concentration
Puro=1.5μg/ml
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS

Product Description

SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control.
Gene Expression Validation
No. Sample SIRT3CтMean h_actin-3CтMean ΔCтMean ΔΔCт Expression Rate
1 SIRT3 Stable Expression Cell Line (NCI-H716) 19.522 15.285 4.237 -5.340 40.504
2 NCI-H716_Ctrl(Puro) 25.978 16.401 9.577 0.000 1.000
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H716		 STR Info (Cell bank)
Cell Line:NCI-H716 [H716]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 811811
D7S820 10111011
D16S539 11121112
VWA 16171617
TH01 69.369.3
AMEL XXXX
TPOX 811811
CSF1PO 11111111
D12S391 1819
FGA 2022
D2S1338 1416
D21S11 32.232.2
D18S51 1516
D8S1179 1515
D3S1358 1618
D6S1043 1213
PENTAE 712
D19S433 1518.2
PENTAD 99
D1S1656 1616
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    High expression
    Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
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    Good stability
    Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
  • icon
    Strict QC
    Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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